翻訳と辞書 Words near each other ・ Nucleation ・ Nucleation in microcellular foaming ・ Nuclei Armati Rivoluzionari ・ Nuclei colliculi superioris ・ Nuclei Territoriali Antimperialisti ・ Nucleic acid ・ Nucleic acid analogue ・ Nucleic acid design ・ Nucleic acid double helix ・ Nucleic acid hybridization ・ Nucleic acid inhibitor ・ Nucleic acid metabolism ・ Nucleic acid methods ・ Nucleic acid nomenclature ・ Nucleic acid notation ・ Nucleic acid quantitation ・ Nucleic acid quaternary structure ・ Nucleic acid secondary structure ・ Nucleic acid sequence ・ Nucleic acid structure ・ Nucleic acid structure determination ・ Nucleic acid structure prediction ・ Nucleic acid templated chemistry ・ Nucleic acid tertiary structure ・ Nucleic acid test ・ Nucleic acid thermodynamics ・ Nucleic Acids Research ・ Nucleo Operativo Centrale di Sicurezza ・ Nucleobase ・ Nucleobase cation symporter-1 Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Nucleic acid quantitation ： ウィキペディア英語版 Nucleic acid quantitation In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. There are several methods to establish the concentration of a solution of nucleic acids, including spectrophotometric quantification and UV fluorescence in presence of a DNA dye. == Spectrophotometric analysis == Nucleic acids absorb ultraviolet light in a specific pattern. In a spectrophotometer, a sample is exposed to ultraviolet light at 260 nm, and a photo-detector measures the light that passes through the sample. The more light absorbed by the sample, the higher the nucleic acid concentration in the sample. Using the Beer-Lambert Law it is possible to relate the amount of light absorbed to the concentration of the absorbing molecule. At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA is 0.020 (μg/ml)−1 cm−1, for single-stranded DNA it is 0.027 (μg/ml)−1 cm−1, for single-stranded RNA it is 0.025 (μg/ml)−1 cm−1 and for short single-stranded oligonucleotides it is dependent on the length and base composition. Thus, an Absorbance (A) of 1 corresponds to a concentration of 50 μg/ml for double-stranded DNA. This method of calculation is valid for up to an A of at least 2. A more accurate extinction coefficient may be needed for oligonucleotides; these can be predicted using the nearest-neighbor model. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Nucleic acid quantitation」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.